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Conservation Genetics of the Endangered Shasta Crayfish (Pacifastacus fortis):
Habitat loss due to hydrologic and agricultural manipulation has been detrimental to the Shasta crayfish population. However, even more harmful has been the introduction of non-native species including bullfrogs, muskrats, many fishes, and most notably, the Signal Crayfish (P. leniusculus). The only native crayfish endemic to California remaining in existence, the Shasta crayfish was State and federally listed in 1988; however, populations continuing to decline. Up to this point, the genetic structure of the Shasta crayfish is unknown. Microsatellite markers have been developed and are being employed to examine the extent of genetic diversity within and between the remaining populations of P. fortis in Shasta County, CA. The information gained from this study will be applied to management of this species in the hopes of protecting it from extinction.
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Population Genetics, Genetics of Aquaculture, and Linkage Mapping of the Pacific Lion-Paw Scallop (Nodipecten subnodosus) Around the Baja California Peninsula, Mexico:
The lion-paw scallop is the largest scallop species found in the tropical waters of the Eastern Pacific Ocean and is found both the Pacific and Gulf of California coasts of the Baja California Peninsula of Mexico. An important aquaculture species, fishery harvest in the Lagoon of Ojo de Liebre increased substantially along with the economic value of the eatible muscle.
Along with Dr. Ana Maria Ibarra of CIBNOR (La Paz, Mexico), microsatellite markers have been developed for population analyses of the lion-paw scallop in natural and aquaculture populations along the coast of the Peninsula. In addition, we have examined the maternal and paternal contribution in induced mass spawn events and are currently working to create a linkage map of the genome with the goal of finding QTL associated with important aquacultural traits.
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Publications
Ibarra, A.M., J.L. Petersen, T.R. Famula, and B.P. May. 2006. Characterization of 35 microsatellite loci in the Pacific lion-paw scallop (Nodipecten subnodosus) and their cross-species amplification in four other scallops of the Pectinidae family. Molecular Ecology Notes: 6:153-156.
Petersen, J.L., R. Bischof, G.L. Krapu, and A.L. Szalanski. 2003. Genetic variation in the Midcontinental population of sandhill crane, Grus canadensis. Biochemical Genetics. 41:1-12.
Szalanski, A.L., G. Steinauer, R. Bischof, and J. Petersen. 2001. Origin and conservation genetics of the threatened ute ladies-tresses, Spiranthes diluvialis (Orchidaceae). Amer. J. Botany 88:177-180.
Petersen, J.L., A.M. Ibarra, J.L. Ramirez, and B.P. May. 2008. An induced mass spawn of the hermaphroditic lion-paw scallop, Nodipecten subnodosus: Genetic assignment of maternal and paternal parentage. Journal of Heredity: 99:337-348.
Fisch, K.M., J.L. Petersen, M.R. Baerwald, J.K. Pedroia, and B.May. 2009. Characterization of 24 microsatellite loci in delta smelt, Hypomesus transpacificus, and their cross-species amplification in two other smelt species of the Osmeridae family. Molecular Ecology Resources: 9:405-408.
Petersen, J.L., A.M. Ibarra, and B. May. 2009. Thirty-seven additional microsatellite loci in the Pacific lion-paw scallop (Nodipecten subnodosus) and cross-amplification in other Pectinids. In press for Conservation Genetics Resources.
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