Judy Callis
Professor
jcallis@ucdavis.edu

Molecular & Cellular Biology

Office
103A Briggs Hall
752-1015

Lab
752-1014


Picture of Judy Callis
 
Degrees:
1987 PhD Stanford University Biology
1981 MA University of Illinois, Urbana-Champaign Botany
1977 BA Washington University Biology
Research Interests:

Specificity of the ubiquitin pathway of regulated proteolysis. Identification and analysis of substrates in the pathway in yeast and higher plants. Studies on the degradation of a hormonally induced higher plant protein important in the auxin signal transduction pathway. Studies on a ubiquitin-like protein, Rub1p, in yeast and in plants involved in cell-cycle control. Studies on ubiquitin carboxy-specific proteases (UBPs); their role in regulating protein degradation. Studies on the RING type E3 ligase family in Arabidopsis thaliana- E2 specificity, linkages, in vivo function, targets.
Awards:
Elected Fellow, American Association for the Advancement of Science 2002
National Science Foundation Presidential Young Investigator Faculty Award, 1991-1996.
NSF Postdoctoral Fellowship in Plant Biology, 1987-1989.
NIH Predoctoral Trainee, 1981-1983.
Paul K. and Ruth R. Stumpf Endowed Chair in Plant Biochemistry
Department and Center Affiliations:
Department of Molecular and Cellular Biology
Secondary Member, Department of Plant Biology
Department of Molecular and Cellular Biology
Secondary Member, Department of Plant Biology
Professional Societies:
American Society for Biochemistry and Molecular Biology
American Society of Plant Biologists
Genetics Society of America
International Society for Plant Molecular Biologists
The American Society for Cell Biologists
CBS Graduate Group Affiliations:
Biochemistry, Molecular, Cellular and Developmental Biology  
Genetics  
Plant Biology  
Publications:
  • Dreher, K.A. and Callis, J. 2007 Ubiquitin, Hormones and Biotic Stress in Plants. Annals of Bot. 9: 787 - 822.
  • Stone SL, Callis J. Ubiquitin ligases mediate growth and development by promoting protein death. Curr Opin Plant Biol. 2007 Sep 10; PMID: 17851112
  • Doelling JH, Phillips AR, Soyler-Ogretim G, Wise J, Chandler J, Callis J, Otegui MS, Vierstra The Ubiquitin-Specific Protease Subfamily UBP3/UBP4 Is Essential for Pollen Development and Transmission in Arabidopsis. Plant Physiol. 2007 Sep 28, PMID: 17905865
  • Scherer GF, Zahn M, Callis J, Jones AM. A role for phospholipase A in auxin-regulated gene expression. FEBS Lett. 2007 581:4205-11
  • Johnson, L.M., Bostick, M. Zhang, X, Kraft, E., Henderson, I., Callis, J. and Jacobsen, S.E. 2007 The SRA Methyl-Cytosine-Binding Domain Links DNA and Histone Methylation. Current Biology 17, 1-6
  • Stone, S.L., Williams, L.A., Farmer, L.M., Vierstra, R.D. and Callis, J. 2006 KEEP ON GOING, a RING E3 ligase essential for Arabidopsis growth and development, is involved in abscisic acid signaling. Plant Cell 18, 3415-3428
  • Dreher, K., Brown, J. Saw, R. and Callis, J. 2006 The Arabidopsis Aux/IAA Protein Family Has Diversified in Degradation and Auxin Responsiveness. The Plant Cell, 18, 699-714.
  • Callis, J. 2005 Plant biology: Auxin action (Invited commentary) Nature 435, 436-437
  • Kraft, E., Stone, S., Ma, L., Su, N., Gao, Y., Lau, O., Deng, X-W, and Callis, J. 2005 Genome Analysis and Functional Characterization of the E2 and RING domain E3 Ligase Ubiquitination Enzymes of Arabidopsis thaliana Plant Physiol., 139, 1597-1611.
  • Callis, J and Ling, R 2005 Preparation, Characterization, and Use of Tagged Ubiquitins. Methods in Enzymol. 399, 51-64
  • Figueroa, P., Gusmaroli, G., Serino, G., Habashi, J., Ma, L., Shen, Y., Feng, S., Bostick, M., Callis, J., Hellmann, H., and Deng, X-W. 2005 Arabidopsis Has Two Redundant Cullin3 Proteins That Are Essential for Embryo Development and that Interact with RBX1 and BTB Proteins to Form Multisubunit E3 Ubiquitin Ligase Complexes in Vivo. Plant Cell 17, 1180-1195
  • Stone, S., Hauksdottir, H., Troy, A., Herschleb, J., Kraft, E., and Callis, J. 2005 Functional analysis of the RING type ubiquitin ligase family of Arabidopsis thaliana L. Plant Physiol., 137: 13-30.
  • Laplaza, J.M.+, Bostick, M.+, Scholes, D.T., Curcio, M.J., and Callis, J. 2004 Saccharomyces cerevisiae ubiquitin-like protein Rub1p conjugates to cullin family members Rtt101p and Cul3p in vivo. Biochemical Journal 377:459-467. +these authors contributed equally to the work.
  • Magnolia Bostick, Stephanie R. Lochhead, Adria Honda, Scott Palmer, and Judy Callis 2004 RUB1 and RUB2 (Related to Ubiquitin) are redundant, essential, and regulate vegetative growth, auxin signaling, and ethylene production in Arabidopsis, Plant Cell 16, 2418-2432
  • Zenser, N., Dreher, K.A. Edwards, S.R., and Callis, J. 2003. Acceleration of Aux/IAA proteolysis is specific for auxin and independent of AXR1 Plant J. 35: 285-294.
  • Yoshida, S., Ito, M., Callis, J., Nishida, I. and Watanabe, A. 2002. A delayed leaf senescence mutant is defective in arginyl-tRNA : protein arginyltransferase, a component of the N-end rule pathway in Arabidopsis. Plant J. 32:129-137.
  • Linghu, B., Callis, J., and Goebl, M. 2002 Rub1p processing by Yuh1p is required for wild-type levels of Rub1p conjugation to Cdc53p. Eukaryotic Cell, 1: 491-494.
  • Ramos, JA, Zenser, N, Leyser, O, and Callis, J 2001 Rapid Degradation of Auxin/Indoleacetic Acid Proteins Requires Conserved Amino Acids of Domain II and Is Proteasome Dependent Plant Cell 13: 2349-2360.
  • Schwechheimer C, Serino G, Callis J, Crosby WL, Lyapina S, Deshaies R, Gray WL, Estelle M and X-W Deng. 2001. Interactions of the COP9 Signalosome with the E3 Ubiquitin Ligase SCFTIR in Mediating Auxin Response. Science 292:1379-1382.
  • Zenser N, Ellsmore A, Leasure C, and J Callis. 2001. Auxin modulates the degradation rate of Aux/IAA proteins. Proceedings of the National Academy of Sciences USA. 98:11795-11800
  • McClinton, R. S.; Chandler, J. S.; Callis, J. 2001 cDNA isolation, characterization, and protein intracellular localization of a katanin-like p60 subunit from Arabidopsis thaliana. Protoplasma . 216 (3-4): 181-190.
  • Callis, J; Vierstra, RD. 2000 Protein degradation in signaling. Current Opinion in Plant Biology 3: 381-6.
  • Worley, CK; Zenser, N; Ramos, J; Rouse, D; Leyser, O; Theologis, A; Callis, J. 2000 Degradation of Aux/IAA proteins is essential for normal auxin signalling. Plant Journal 21: 553-62.
  • Ling, R; Colon, E; Dahmus, ME; Callis, J. 2000 Histidine-tagged ubiquitin substitutes for wild-type ubiquitin in Saccharomyces cerevisiae and facilitates isolation and identification of in vivo substrates of the ubiquitin pathway. Analytical Biochemistry 282: 54-64.
  • Rao-Naik, C; Chandler, JS; McArdle, B; Callis, J. 2000 Ubiquitin-specific proteases from Arabidopsis thaliana: cloning of AtUBP5 and analysis of substrate specificity of AtUBP3, AtUBP4, and AtUBP5 using Escherichia coli in vivo and in vitro assays. Archives of Biochemistry and Biophysics 379: 198-208.
  • Callis, J; Vierstra, RD. 2000 Protein degradation in signaling. Current Opinion in Plant Biology 3: 381-6.
Laboratory Personnel:
Briggs Hall Room 103
http://www.mcb.ucdavis.edu/faculty-labs/Callis/
see our web page for current and past members!

Teaching Interests:
Biochemistry- I teach BIS103, called Bioenergetics and metabolism. I co-teach Plant Biochemistry. I also teach an undergraduate seminar in Biochemistry/Molecular Biology. I give lectures in several graduate level courses in Plant Biology.
Courses:
BIS 103 Energetics and Metabolism Winter,Spring
MCB 138 Undergraduate Seminar Winter
MCB 126 Plant Biochemistry Winter,Spring