Enoch Post Baldwin
Associate Professor
epbaldwin@ucdavis.edu

Molecular & Cellular Biology
Chemistry

Office
4B Briggs
752-1108

Lab
752-5235


Picture of Enoch P. Baldwin
 
Degrees:
1989 PhD University of California, Berkeley Chemistry
1982 BA State University of New York, Purchase Chemistry
Research Interests:
Structural Biochemistry, Enzymology and Protein Engineering of Protein-nucleic acid Interactions
X-ray crystallography, protein and DNA structure and function, and protein engineering, focusing on enzymology and molecular recognition. Model systems include DNA and nucleotide binding proteins and enzymes.
My lab uses X-ray crystallography, biochemistry and molecular genetics to dissect and engineer mechanisms of enzymes involved in nucleic acid metabolism. A primary focus is understanding the basis for the DNA specificity of Cre site-specific recombinase, and using this understanding to obtain variants that recognize alternative DNA sequences. A second focus is the mechanism and regulation of Cytidine Triphosphate Synthetase, the ultimate enzyme for pyrimidine biosynthesis, and a target for anti-cancer and anti-parasitic drugs.
Awards:
2006, "Teacher of the Year", Biochemistry and Molecular Biology Graduate Group
Department and Center Affiliations:
Department of Chemistry
Molecular and Cellular Biology
Professional Societies:
American Chemical Society
Protein Society
AAAS
CBS Graduate Group Affiliations:
Biochemistry, Molecular, Cellular and Developmental Biology  
Biophysics  
Graduate Groups not Housed in CBS:
Microbiology  
Chemistry  
Publications:
  • Gelato, K.A, Martin, S.S, Liu, P.H., Saunders, A.A., and E.P. Baldwin. 2008. Spatially-Directed Assembly of a Heterotetrameric Cre-Lox Synpase Restricts Recombination Specificity. J. Mol Biol. 385, 653-665.
  • Chang, Y.F., Martin, S.S., Baldwin, E.P., & Carman, G.M. 2007. Phosphorylation of Human CTP Synthetase 1 by Protein Kinase C: Identification of Ser462 and Thr455 as major sites of phosphorylation. J. Biol. Chem. 282, 17613-22.
  • Gelato, K.A., Martin, S.S., Wong, S., and Baldwin, E.P. 2006. Mulitple Levels of Affinity-Dependent DNA Discrimination in Cre-LoxP Recombination. Biochemistry 45, 12216-26.
  • Gelato, K.A., Martin, S.S., Baldwin, E.P. 2005. Reversed DNA Strand Cleavage Specificity in Initiation of Cre-LoxP Recombination Induced by the His289Ala Active Site Substitution. J. Mol. Biol. 354, 233-45.
  • Endrizzi, J.A., Kim, H., Anderson, P.M., Baldwin, E.P. 2005. Mechanisms of product feedback regulation and drug resistance in cytidine triphosphate synthetases from the structure of a CTP-inhibited complex. Biochemistry 44, 13491-9.
  • Han, G.-S. , Sreenivas, A., Choi, M.-G. , Chang, Y.-F., Martin, S.S. , Baldwin, E.P., and G. M. Carman. 2005. Expression of Human CTP Synthetase in Saccharomyces cerevisiae Reveals Phosphorylation by Protein Kinase A, J. Biol. Chem. 280, 38328-36.
  • Endrizzi, J.A., Kim, H., Anderson, P.M., Baldwin, E.P. 2004. Crystal Structure of Escherichia coli Cytidine Triphosphate Synthetase, a Nucleotide-Regulated Glutamine Amidotransferase/ATP-Dependent Amidoligase Fusion Protein and Homologue of Anticancer and Antiparasitic Drug Targets. Biochemistry 43, 6447-63.
  • Friddle, RW, Klare, JE, Martin, SS, Corzett, M, Balhorn, R, Baldwin, EP, Baskin, RJ, and A Noy. 2004. Mechanism of DNA compaction by yeast mitochondria protein ABF2p. Biophysical J. 86, 1632-9.
  • Baldwin EP, Martin SS, Abel J, Gelato KA, Kim H, Schultz PG, and SS Santoro. 2003. A specificity switch in selected Cre recombinase variants is mediated by macromolecular plasticity and water. Chem. Biol. 110, 1085-93.
  • Martin SS, Wachi S, and EP Baldwin. 2003. Vanadate-based transition-state analog inhibitors of Cre-LoxP recombination. Biochem. Biophys Res. Comm. 308: 529-34.
  • Brewer, LR, Friddle, R, Noy, A, Baldwin, E, Martin, SS, Corzett, M, Balhorn, R and RJ Baskin. 2003. Packaging of Single DNA Molecules by the Yeast Mitochondrial Protein ABF2p. Biophysical J. 85, 2519-24.
  • Martin SS, Chu VC, and EP Baldwin. 2003. Modulation of active complex assembly and turnover rate by protein-DN Interactions in Cre-LoxP recombination. Biochemistry 42: 6814-6826.
  • Martin SS, Pulido E, Chu, VC, Lechner TS, and EP Baldwin. 2002. The order of strand exchanges in Cre-LoxP recombination and its basis suggested by the crystal structure of a Cre-LoxP Holliday junction complex. J. Mol. Biol. 319: 107-127.
  • Denison, MS, Pandini, A, Nagy, SR, Baldwin, EP, and Bonati, L. 2002. Ligand binding and activation of the Ah receptor. Chem. Biol. Interact. 141: 3-24.
  • Woods KC, Martin SS, Chu VC and EP Baldwin. 2001. Quasi-equivalence in site-specific recombinase structure and function:crystal structure and activity of trimeric Cre recombinase bound to a three-way Lox DNA junction. J. Mol. Bio. 313:49-69
Laboratory Personnel:
Baldwin Lab
http://www-crystal.ucdavis.edu/talent/baldwinlab/index.html
Shelley Martin (1997-2007), Kathy Gelato (2001-2007), Hanseong Kim (2001-2005), Dr. James Endrizzi (2002-2006), Lesley Steinman (2004-2007), Roger Jessinghaus (2008-)

Teaching Interests:
Macromolecular structure and function, macromolecular interactions, protein sequence-structure-function relationships, enzyme mechanisms, X-ray structure determination.
Courses:
BMB 221A Physical Biochemistry (7 Lectures) Fall
BMB 221C Molecular Biology (3 Lectures) Spring
BMB 221B Mechanistic Enzymology (every other year) Fall
MCB 124 Macromolecular Structure and Function Fall
Research Contribution to Society:
Through the understanding of protein function at the atomic level, we can better target therapeutic strategies for proteins of medical interest by 1) re-engineering function; 2) focused efforts for developing small molecule ligand modulators, and 3) ascertaining their structural and functional relationships to other macromolecules.
Key Words:
Enzyme Structure and Function; X-ray Crystallography; Protein Engineering; In-vitro Evolution; Cre recombinase; Site-specific recombination; Nucleotide Biosynthesis and Regulation; Structure-based Ligand Discovery