Francis J. McNally
Offices and Labs
|1990||PhD||Biochemistry||University of California, Berkeley|
|1984||BA||Biochemical Sciences||Princeton University|
Mechanisms of Asymmetric Cell Division during Female Meiosis
Meiosis is a process by which animals, plants and fungi reduce chromosome number four-fold to produce haploid gametes. In females of both plants and animals, only one of four sets of chromosomes segregated during meiosis is inherited by a gamete. In animals, this asymmetric inheritance is mediated by asymmetric meiotic spindle positioning and highly asymmetric cell divisions that yield a large haploid egg. We are interested in the mechanisms that mediate these asymmetric divisions and utilize C. elegans because both meiotic divisions can be filmed in utero using fluorescent protein fusions and time-lapse imaging. We use a combination of classical genetics, RNAi and biochemistry to elucidate the roles of proteins that regulate microtubule-based motility, microtubule organization and microtubule dynamics in meiotic cell division.
Department and Center Affiliations
CBS Grad Group Affiliations
Specialties / Focus
- Cell Division and the Cytoskeleton
- Chromosome Dynamics and Nuclear Function
- Cell Biology
- Developmental Biology
- Reproductive Biology
Crowder ME, Flynn JR, McNally KP, Cortes DB, Price KL, Keuhnert PA, Panzica MT, Andaya A, Leary JA, McNally FJ. 2015. Dynactin-dependent cortical dynein and spherical spindle shape correlate temporally with meiotic spindle rotation in C. elegans. Mol Biol Cell. 26: 3030-3046.
Cortes DB, McNally KL, Mains PE, McNally FJ. 2015. The asymmetry of female meiosis reduces the frequency of inheritance of unpaired chromosomes. eLife 2015;4:e06056.
McNally K, Berg E, Cortes DB, Hernandez V, Mains PE, McNally FJ.(2014).Katanin maintains meiotic metaphase chromosome alignment and spindle structure in vivo and has multiple effects on microtubules in vitro. Mol Biol Cell. 25:1037-49.
McNally FJ. (2013). Mechanisms of spindle positioning. J Cell Biol. 200:131-40.
McNally, K., Fabritius, A.S., Ellefson, M.L., Flynn, J.R., Milan, J.A. and McNally, F.J. (2012) Kinesin-1 prevents capture of the oocyte meiotic spindle by the sperm aster. Developmental Cell 22: 788-789.
O'Donnell L, Rhodes D, Smith SJ, Merriner DJ, Clark BJ, Borg C, Whittle B, O'Connor AE, Smith LB, McNally FJ, de Kretser DM, Goodnow CC, Ormandy CJ, Jamsai D, O'Bryan MK. (2012). An essential role for katanin p80 and microtubule severing in male gamete production.PLoS Genet. 2012;8(5):e1002698
Ellefson ML, McNally FJ. (2011) CDK-1 inhibits meiotic spindle shortening and dynein-dependent spindle rotation in C. elegans. J Cell Biol. 193:1229-44.
Fabritius AS, Flynn JR, McNally FJ. (2011). Initial diameter of the polar body contractile ring is minimized by the centralspindlin complex. Dev Biol. 359:137-48.
Loughlin R, Wilbur JD, McNally FJ, Nédélec FJ, Heald R. (2011). Katanin contributes to interspecies spindle length scaling in Xenopus. Cell. 147:1397-407.
Fabritius, A.S., Ellefson, M.L., and McNally, F.J. 2011. Nuclear and spindle positioning during oocyte meiosis. Curr Opin Cell Biol. In press.
McNally KP, McNally FJ. (2011) The spindle assembly function of Caenorhabditis elegans katanin does not require microtubule-severing activity. Mol Biol Cell. 22:1550-60.
McNally, K.L., Martin, J.L., Ellefson, M., McNally, F.J. 2010. Kinesin-dependent transport results in polarized migration of the nucleus in oocytes and inward movement of yolk granules in meiotic embryos. Dev. Biol. 339: 126-140.
Roll-Mecak, A., McNally, F.J. 2010. Microtubule-severing enzymes. Curr. Opin. Cell Biol. 22: 96-103.
Ellefson, M.L., and F.J. McNally. 2009. Kinesin-1 and cytoplasmic dynein act sequentially to move the meiotic spindle to the oocyte cortex in Caenorhabditis elegans. Mol. Biol. Cell 20: 2722-2730.
Meyerzon, M., Fridolfsson, H.N., Ly, N., McNally, F.J., Starr, D.A. 2009. UNC-83 is a nuclear-specific cargo adaptor for kinesin-1-mediated nuclear migration. Development. 136: 2725-2733.
Johnson, J.L., Lu, C., Raharjo, E., McNally, K., McNally, F.J., Mains, P.E. 2009. Levels of the ubiquitin ligase substrate adaptor MEL-26 are inversely correlated with MEI-1/katanin microtubule-severing activity during both meiosis and mitosis. Dev Biol. 330: 349-357.
McNally, K., Audhya, A., Oegema, K., McNally, F.J. 2006. Katanin controls mitotic and meiotic spindle length. J. Cell Biol. 175: 881-891.
Yang, H., Mains, P.E., and F.J. McNally. 2005. Kinesin-1 mediates translocation of the meiotic spindle to the oocyte cortex through KCA-1, a novel cargo adapter. J. Cell Biol. 169: 447-457.
McNally, K.L., and F.J. McNally. 2005. Fertilization initiates the transition from anaphase I to metaphase II during C. elegans meiosis. Dev. Biol. 282: 218-230.
Yang, H., McNally, K., and F.J. McNally. 2003. MEI-1/Katanin is Required for Translocation of the Meiosis I Spindle to the Oocyte Cortex in C. elegans. Dev. Biol. 260: 245-259..