Roy H. Doi

image of Roy H. Doi

Professor Emeritus


Molecular & Cellular Biology


0 AA 1951 Placer Junior College
0 BA Physiology - 1953 University of California, Berkeley
0 BA Bacteriology - 1957 University of California, Berkeley
0 PhD Bacteriology - 1960 University of Wisconsin, Madison
0 MS Bacteriology - 1958 University of Wisconsin, Madison

Research Interests

Structure, function, and assembly of Clostridium cellulovorans cellulosome (cellulase).


Senior US Scientist Award, Alexander von Humboldt Foundation, 1978-1979
Fellow , American Association for the Advancement of Science, 1988.
Member, Recombinant DNA Advisory Committee (RAC) of the National Institutes of Health, 1990-1994.
Editor, Microbiology and Molecular Biology Reviews, 1998-2006
Eminent Scientist of Riken, Riken Institute, Wako, Japan, 1998.
Fellow, American Academy of Microbiology, 2001.
Distinguished Professor, UC Davis, 2003-present
Member, National Academy of Sciences, 2006

Department and Center Affiliations

Molecular & Cellular Biology


American Society for Microbiology

Graduate Groups not Housed in CBS


Field Sites


Teaching Interests

Biochemistry. Bioenergetics and metabolism. Molecular biology. Gene expression.


5/21/2010 9:26:34 AM
  • Arai, T., Matsuoka, S., Cho, H.-Y., Yukawa, H., Inui, M., Wong, S.-L., and Doi, R.H. Synthesis of Clostridium cellulovorans minicellulosomes by intercellular complementation. Proc. Natl. Acad. Sci. USA 104:1456-1460 (2007).
  • Arai, T., Kosugi, A., Chan, H., Koukiekolo, R., Yukawa, H., Inui, M. and Doi, R.H. Properties of cellulosomal family 9 cellulases from Clostridium cellulovorans. Appl. Microbiol Biotechnol. 71: 654-660 (2006).
  • Kosugi, A., Arai, T. and Doi, R.H. Degradation of cellulosome-produced cello- oligosaccharides by an extracellular non-cellulosomal ²-glucan glucohydrolase, BglA, from Clostridium cellulovorans. Biochem. Biophys. Res. Commun. 349: 20-23 (2006).
  • Murashima, K., Kosugi, A., and Doi, R.H. Site-directed mutagenesis and expression of the soluble form of the family IIIa cellulose binding domain from the cellulosomal scaffolding protein of Clostridium cellulovorans. J. Bacteriol. 187:7146-7149 (2005).
  • Han, S.-O., Yukawa, H., Inui, M. and Doi, R.H. Molecular cloning, transcriptional and expression analysis of engO, encoding a non noncellulosomal family 9 enzyme from Clostridium cellulovorans. J. Bacteriol. 187:4884-4889 (2005).
  • Doi, R.H. and Kosugi, A. Cellulosomes: Plant Cell Wall Degrading Enzyme Complexes. Nature Reviews - Microbiology 2: 541-551 (2004).
  • Cho, H.-Y., Yukawa, H., Inui, M., Doi, R.H. and Wong, S.-L, Production of minicellulosomes from Clostridium cellulovorans in Bacillus subtilis WB800. Appl. Environ. Microbiol. 70:5704-5707 (2004).
  • Kosugi, A., Amano, Y. Murashima, and Doi, R.H. Hydrophjilic domains of scaffolding protein CbpA promote glycosyl hydrolase activity and localization of cellulosomes to the cell surface of Clostridium cellulovorans. J. Bacteriol. 186:6351-6359 (2004).
  • Han, S.-O., Cho, H.-Y., Yukawa, H., Inui, M. and Doi, R.H. Regulation of expression of cellulosomes and non-cellulosomal (hemi-)cellulolytic enzymes in Clostridium cellulovorans during growth on different carbon sources. J. Bacteriol. 186:4218-4227 (2004).