Wolf-Dietrich Heyer

image of Wolf-Dietrich Heyer

Professor and Chair

Departments

Microbiology and Molecular Genetics

Offices and Labs

3163 Life Sciences
Tel. 752-3001

Degrees

1995 Habilitation Microbiology & Molecular Genetics University of Bern (Switzerland)
1985 PhD Biology University of Bern. Switzerland
1982 Diploma Biology Free University of Berlin (Germany)

Research Interests

Mechanism and regulation of recombinational DNA repair

Double-stranded DNA breaks (DSBs) are among the most genotoxic lesions and can be generated by ionizing radiation, drugs, or cellular processes. In eukaryotes, several pathways compete for the repair of such lesions. Homologous recombination is the critical DSB repair pathway in yeasts and an important DSB repair pathway in all eukaryotes studied. We are using  Saccharomyces cerevisiae as a lead system and employ genetical, molecular, and biochemical methods to elucidate the molecular mechanism of homologous recombination and recombinational DNA repair and its regulation by the DNA damage checkpoints. The key results are validated with human proteins to establish lasting paradigms. Recombination has dual signfiicance for cancer. Recombination genes are guardians of the genome and classic tumor suppressors. Hence, defects in recombination genes increase cancer risk. DNA repair and specifically recombination is also critical in anti-cancer therapy, as many modalities of treatment involve DNA damaging agents. We are applying our mechanistic insights from basic studies in more translational studies relating to cancer predispostion and anti-cancer therapy.

Awards

1989 Richard A. Smith Award for Outstanding Scientific Achievement (Dana-Farber Cancer Institute, Harvard Medical School, USA)
1995 Eduard-Adolf-Stein Award for the best Habilitation at the Philosophical-Natural Sciences Faculty of the University of Bern (CH) 1990-1995
1997 Theodor-Kocher Price for the best "Young Researcher at the University of Bern 1997"
2000 Chancellor's Fellow 2000-2005, University of California, Davis
2002 , 2009 Outstanding Teaching Award, Biochemistry and Molecular Biology Graduate Students
2010 Rothschild-Yvette Mayent-Institut Curie Award, Institut Curie, Paris (F)
2011 UC Davis Chancellor's Award for Excellence in Mentoring Undergraduate Research
2011 College of Biological Sciences (UC Davis) Faculty Research Award
2012 Fellow, American Academy of Microbiology

Department and Center Affiliations

Department of Microbiology & Molecular Genetics
Department of Molecular & Cellular Biology
UC Davis Comprehensive Cancer Center

ProfessionalSocieties

American Association for the Advancement of Science (AAAS)
Genetics Society of America
American Society of Microbiology
American Society for Biochemistry and Molecular Biology
Deutscher Hochschulverband
Gesellschaft fuer Biochemie und Molekularbiologie

CBS Grad Group Affiliations

Biochemistry, Molecular, Cellular and Developmental Biology
Integrated Genetics and Genomics

Specialties / Focus

Integrated Genetics and Genomics
  • Chromosome Biology
Biochemistry, Molecular, Cellular and Developmental Biology
  • Cell Biology
  • Cell Division and the Cytoskeleton
  • Chromosome Dynamics and Nuclear Function

Graduate Groups not Housed in CBS

Microbiology
Pharmacology and Toxicology (PTX)

Labs

Our laboratory is located in LS 3243, LS 3249, LS 3144, LS 3116. My office is LS 3165. website
  • The members of my group are: Rita Alexeeva, Dr. Paula Cerqueira, Dr. Hang Phuong Le, Dr. Jie Liu, Dr. Aurèle Piazza, Dr. João Vieira, Dr. William Wright, Steven Gore, Shirin Jenkins, Shanaya Shah, Noelle Cabral, Rudy Gallegos, Rupleen Kaur, Sreevidya Kurra, Kartik Singhal, Jorge Vaquero, Jeff Bellinder.

Teaching Interests

Yeast physiology and genetics. Molecular genetics. DNA metabolism.

Courses

MIC 275 Seminar in DNA Repair and Recombination
GGG 295 Seminar in Molecular Genetics (Fall (biannual, even years))
MIC 276 Adv Concepts DNA Metab (Winter (biannual, even years))
BCB 214 Molecular Biology (Spring)

Publications

7/26/2016 2:27:18 PM
  • McVey, M., Khodaverdian, V.Y., Meyer, D., Cerqueira, P.G., and Heyer W.-D. (2016) Eukaryotic    DNA polymerases in homologous recombination. Annu. Rev. Genet. In Press.

     

  • Ganai, R.A., Zhang, X., Heyer, W.-D., and Johansson, E. (2016) Strand displacement synthesis by yeast DNA polymerase ε, Nucleic Acids Res. 2016 Jun 20. pii: gkw556. [Epub ahead of print].

  • Spies, J., Waizenegger, A., Barton, O., Sürder, M., Wright, W.D., Heyer, W.-D., and Löbrich, M. (2016) Nek1 phosphorylates Rad54 in G2 phase to promote homologous recombination and prevent replication fork instability. Mol. Cell 62, 903-917.

  • Janke, R., Kong, J., Braberg, H., Cantin, G., Yates III, J., Krogan, NJ, and Heyer, W.-D. (2016) Nonsense-mediated decay regulates key components of homologous recombination. Nucleic Acids Res. 44, 5218-5230.

  • Crawley, J.N., Heyer, W.-D., and LaSalle, J.M. (2016) Autism and cancer share risk genes, pathways, and drug-targets. Trends in Genetics 32,139-146.

  • Jenkins, S.S., Mukherjee, S., and Heyer, W.-D. (2016) DNA repair by homologous recombination. In: Ralph A Bradshaw and Philip D Stahl (Editors-in-Chief), Encyclopedia of Cell Biology, Vol 1, Waltham, MA: Academic Press, pp. 456-467.

  • Kowalczykowski, S.C., Hunter, N., and Heyer, W.-D. (2015) DNA Recombination. Perspectives in Biology, Cold Spring Harbor Press.

  • Meyer, D., Fu, B.X.H., and Heyer, W.-D. (2015) DNA Polymerases delta and lambda cooperate in repairing double-strand breaks by Microhomology Mediated End-joining in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 112, 6907-6916.

  • Heyer W.-D. (2015) Regulation of Recombination and Genome Maintenance. doi:10.1101/cshperspect.a016501  In: DNA Recombination. Perspectives in Biology, Cold Spring Harbor Press. Eds. Kowalczykowski, S.C., Hunter, N., and Heyer, W.-D.

  • Mason, J.M., Dusad, K., Wright, W.D., Grubb, J., Budke, B., Heyer, W.-D., Connell, P.P. Weichselbaum, R.R., and Bishop, D.K. (2015) RAD54 family translocases counter genotoxic effects of RAD51 in human tumor cells. Nucleic Acids Res. 43, 3180-3196.

  • Fasching, CL., Cejka, P., Kowalczykowski, S.C., and Heyer W.-D. (2015) Top3-Rmi1 dissolve Rad51-mediated D-loops by a topoisomerase-based mechanism. Mol. Cell 57, 595-606.

     

    [Highlighted in accompanying Minireview: Haber, J.E. (2015) TOPping off Meiosis. Mol. Cell 57, 577-581.]

    [Highlighted in Faculty of 1000 http://f1000.com/prime/725364302?bd=1]

     

  • Mukherjee, S., Wright, W.D., Ehmsen, K.T., and Heyer, W.-D. (2014) The Mus81-Mms4 structure-selective endonuclease undergoes nicked junction-induced conformational changes to bend its DNA substrates for cleavage. Nucleic Acids Res. 42, 6511-6522.

  • Wright, W.D. and Heyer, W.-D. (2014) Rad54 is a Heteroduplex DNA Pump Modulated by Its DNA Substrates and Rad51 during D-loop Formation in Homologous Recombination. Mol. Cell 53, 420-432.

    [Highlighted in Faculty of 1000 http://f1000.com/prime/718262173#recommendations]

  • Sneeden, J.L., Grossi, S.M., Tappin, I., Hurwitz G. and Heyer W.-D. (2013) Reconstitution of recombination-associated DNA synthesis with human proteins. Nucleic Acids Res. 41, 4913-4925.
  • Zinovyev, A., Kuperstein, I., Barillot, E., and Heyer, W.-D. (2013) Synthetic lethality between gene defects affecting a single non-essential molecular pathway with reversible steps. PLoS Computat. Biol. 9(4):e1003016.
  • Zhang, X.-P., Janke, R., Kingsley, J., Luo, J., Fasching, C.L., Ehmsen, K.T., and Heyer, W.-D. (2013) A conserved sequence extending motif III of the motor domain in the Snf2-family DNA translocase Rad54 is critical for ATPase activity. PLoS ONE 8(12): e82184.
  • Munoz-Galvan, S., Tous, C., Gonzalez-Blanco, M., Schwartz, E.K., Ehmsen, K.T., West, S.C., Heyer, W.-D., and Aguilera, A. (2012) Differential roles of Mus81-Mms4, Yen1, and Slx4 in the repair of replication-born double-strand breaks by sister chromatid exchange. Mol. Cell. Biol. 32, 1592-1603.
  • Schwartz, E., Wright, W., Ehmsen, K.T., Evans, D., Stahlberg, H., and Heyer, W.-D. (2012) Mus81-Eme1 functions as single hetero-dimeric endonuclease. Mol. Cell. Biol. 32, 3065-3080.

    {Highlighted as Spotlight Article of Significant Interest Selected from This Issue by the Editors]

  • Liu, J. and Heyer, W.-D. (2011) Who’s who in human recombination: BRCA2 and RAD52. Proc. Natl. Acad. Sci. USA 108, 441-442.
  • Liu, J., Renault, L., Veaute, X., Fabre, F., Stahlberg, H., and Heyer, W.-D. (2011) Rad51 paralogs Rad55-Rad57 balance the anti-recombinase function of Srs2 in Rad51 filament formation. Nature 479, 245-248.

    [Must read in Faculty of 1000 http://f1000.com/13398957]

  • Liu, J., Doty, T., Gibson, B., and Heyer, W.-D. (2010) Human BRCA2 promotes filament formation RPA-covered ssDNA. Nature Structural & Molecular Biology 17, 1260-1262.

    [News and Views by Zou, L. (2010) A protein giant in its entirety. Nature 467, 667-678.]

  • Heyer, W.-D., Ehmsen, K.T., and Li, J. (2010) Regulation of homologous recombination in eukaryotes. Annu. Rev. Genetics. 44, 113-139.
  • Li, X., Stith, C.M., Burgers, P.M., and Heyer, W.-D. (2009) PCNA is required for initiating recombination-associated DNA synthesis by DNA polymerase δ. Mol. Cell 36, 704-713.